Rapid low-cost SNP detection using multiplex one-pot LAMP-OSD assays
This innovative solution offers rapid, low-cost SNP detection using multiplex one-pot LAMP-OSD assays. It enhances traditional LAMP technology for high-accuracy diagnostics with single endpoint visual readout, suitable for pathogen and human gene analysis without complex instruments.
Overview
The multiplex one-pot LAMP-OSD assay is a cutting-edge solution for single nucleotide polymorphism (SNP) detection. This technology leverages the power of loop-mediated isothermal amplification (LAMP) and oligonucleotide strand displacement (OSD) to provide a rapid, low-cost, and highly accurate diagnostic tool. By employing engineered LAMP polymerases and optimized OSD probes, the system produces a visual readout that is both simple and effective, eliminating the need for complex real-time reaction monitoring equipment. This solution has been successfully demonstrated for pathogen and human gene analysis, including direct sample testing using saliva and skin scrapings.
Technical specifications
- Engineered LAMP Polymerases: In-house developed for robust multiplexing.
- OSD Probes: Designed for toehold-mediated strand displacement hybridization, enabling specific signal generation only in the presence of target amplicons.
- Single Enzyme System: Unlike multi-component CRISPR-based methods, this system is cost-effective and modularly engineerable.
- Ready-to-Use Kits: Dried LAMP-OSD reaction mixes provide convenience and ease of use.
- Visual Readout Options: Fluorescence or colorimetric signals can be configured based on partner preferences.
Technology readiness level
The LAMP-OSD technology is currently at TRL 5. It has been validated in a relevant environment with synthetic templates, and further validation is planned with human genomic DNA and biospecimens to benchmark performance against PCR and sequencing.
About University of Texas at Austin
The University of Texas at Austin is a comprehensive public research university and the academic flagship for the state, combining scale with a collaborative, entrepreneurial culture. Industry engages through a dedicated research campus with co‑located laboratories and testbeds, plus a research park that supports company residence, prototyping, and joint development. Companies also access an advanced supercomputing center for data‑intensive work and benefit from integration with a medical school and its clinical practice for translational studies. Research is supported by competitive federal funding from NSF, NIH, DOE, DoD, and NASA. A dedicated technology transfer office streamlines IP, licensing, and startup formation, with experienced staff who work routinely with corporate R&D.
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