Partition-based digital PCR assay for salmonella detection in chocolate
A robust digital PCR assay capable of detecting Salmonella in chocolate, overcoming PCR inhibitors, and providing reliable results within eight hours.
Overview
This cutting-edge digital PCR assay offers a breakthrough in detecting Salmonella in chocolate products. Unlike traditional assays, this partition-based system effectively mitigates the interference of PCR inhibitors commonly found in high-fat food matrices. The assay is capable of detecting as low as one colony-forming unit (CFU) of Salmonella in 375 grams of chocolate within an eight-hour timeframe. This innovation not only promises enhanced reliability and reproducibility but also reduces the need for extensive DNA purification processes.
Technical specifications
- Partition-based digital PCR technology: Segregates PCR inhibitors and non-target DNA into distinct partitions, ensuring consistent assay performance.
- Minimal processing requirements: Utilizes crude DNA extracts obtained via a simple boil-lysis extraction protocol.
- Flexible sample enrichment: Supports 1:4 and 1:6 sample dilutions, enriched at 35°C, with sampling at 5, 6, and 7-hour intervals.
- High sensitivity and specificity: Capable of detecting a single CFU of Salmonella per 375 g of chocolate.
Technology readiness level
The technology is currently at TRL 3, having been experimentally validated in a laboratory setting. Future validation plans include further testing with various chocolate types and Salmonella serotypes to ensure comprehensive applicability across different scenarios.
About Florida State University
Florida State University is a comprehensive public research university in Tallahassee serving a large, diverse student body and combining broad academic breadth with intensive research. Industry engages through a research and technology park adjacent to campus that co‑locates university labs with companies and offers shared core facilities and high‑performance computing. The College of Medicine’s statewide clinical partnerships enable translational collaborations and community‑based studies. Research is supported by competitive federal funding from the National Science Foundation, National Institutes of Health, Department of Energy, and Department of Defense, plus State of Florida and industry support. A dedicated technology transfer office manages IP, licensing, and startup formation, with incubator and prototyping space available in the park.
Inquire about this partnering listing
Industry applications
delivered to your inbox.