Multiplexed detection and quantification of postbiotic compounds

Overview

This solution offers a comprehensive analytical platform for screening, identifying, and quantifying postbiotic compounds in plant-based fermentations. Postbiotics are bioactive molecules produced during fermentation, including vitamins, organic acids, peptides, amino acid derivatives, and polyphenol breakdown products, that contribute to gut health, immune function, and overall wellness. The offering combines two complementary capabilities: high-resolution laboratory-based profiling using nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography-mass spectrometry (LC-MS), plus field-deployable detection through enzyme-based colorimetric assays integrated into a portable, fully automated device. This dual approach enables both rigorous compound discovery and routine, point-of-need quantification of 10+ key postbiotic metabolites.

Technical specifications

Laboratory-based profiling capabilities:

• NMR spectroscopy at 700 MHz capable of quantifying more than 70 abundant metabolites above 10 µM concentration
• LC-MS/MS assays using a Qtrap5500 instrument capable of profiling 500+ metabolites including amino acids and derivatives, biogenic amines, organic acids, indoles, and polyphenol breakdown products
• Established assays for organic acids such as acetate, butyrate, propionate, and lactate
• ISO-17025 certified analytical workflows

Portable detection platform:

• Enzyme-based colorimetric assays for metabolite detection and quantification
• Fully automated, portable device designed for multiplexed, point-of-care metabolite sensing
• Capacity to detect and quantify approximately 10 key postbiotic metabolites simultaneously
• Existing colorimetric redox assay technology adaptable to new postbiotic targets

Infrastructure and expertise:

• Research team of more than 60 staff
• High-resolution LC-MS and NMR instrumentation
• Dedicated device engineering facilities with trained engineers
• Proven track record of deploying portable metabolite detection devices

Technology readiness level

The underlying NMR and LC-MS assay methodologies are validated and routinely used for characterizing hundreds of metabolites in foods, beverages, and plant extracts. The colorimetric assay and portable device platform has been previously developed, deployed, and demonstrated for multiplexed metabolite detection. The proposed 8-month project will adapt existing validated assays to specifically target postbiotic metabolites identified in plant-based fermentation samples, then refine and expand colorimetric assays for the 10 most important compounds and integrate them into the existing portable sensor device. Total project cost is estimated at $75,000. Upon completion, the platform will be ready for further validation, licensing, or co-development with industry partners in the functional food, nutraceutical, or fermentation sectors.