Micro-libs technology for non-destructive ploidy detection in wheat production
Onteko Inc presents a micro-LIBS-based solution for non-destructive ploidy discrimination in wheat DH production. This technology uses laser-induced plasma analysis to distinguish haploid and diploid samples without sample preparation, enhancing efficiency in plant breeding.
Overview
Onteko Inc introduces a cutting-edge solution using micro-LIBS (micro-Laser-Induced Breakdown Spectroscopy) to non-destructively detect ploidy levels in wheat DH production. This innovative approach eliminates the need for sample preparation, DNA extraction, or nuclei isolation, offering a rapid and efficient method for distinguishing between haploid and diploid samples. The technology has already shown significant potential in active commercial settings, making it a viable option for enhancing plant breeding processes.
Technical specifications
Micro-LIBS technology functions by firing a focused laser pulse on biological tissue, creating a plasma. The resulting elemental spectrum is analyzed in milliseconds, allowing for real-time ploidy discrimination. By utilizing supervised multivariate classifiers such as PCA combined with SVM or PLS-DA, the system provides binary haploid/diploid calls per sample. This method is supported by successful discrimination of seed genotypes in commercial applications and peer-reviewed studies.
Technology readiness level
Currently at Technology Readiness Level 3, this technology has been validated through statistically significant spectral discrimination in seed genotype experiments and is poised for further validation phases. Future engagements will focus on feasibility studies and optimization to ensure robustness and scalability in practical applications.
About Onteko Inc
Onteko Inc. is a Mississippi-based startup specializing in advanced laser technology applications. The company develops proprietary laser-based solutions, including high-resolution Laser-Induced Breakdown Spectroscopy (LIBS) spectrometers and innovative laser pulse technology that utilizes burst-mode structures to enhance ionization and detection capabilities. Their work spans diverse sectors, such as developing laser techniques for agricultural efficiency, including nopal de-spining and foliar application methods, as well as specialized laser equipment for medical diagnostics and industrial materials analysis. By crafting inventive solutions and leveraging intellectual property, the team focuses on creating disruptive technologies that address specific industry bottlenecks while providing consulting services for laser process development, safety, and system optimization.
These solutions are designed to address critical inefficiencies in fields like agriculture, healthcare, and manufacturing. For instance, their laser technologies offer precise, non-invasive methods for plant care and medical sampling, aiming to improve safety, reduce waste, and enhance performance compared to traditional processes. The company actively engages in research, development, and technical consulting, utilizing a deep expertise in laser science to help clients navigate complex technical challenges, improve existing laser systems, and implement new, specialized applications. Through a combination of hardware development and process innovation, Onteko seeks to drive technological progress and foster seamless technology transfer across various international industries.
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