Arms-based SNP detection for micronutrient deficiency risk
A rapid, cost-effective ARMS-PCR solution for detecting SNPs linked to micronutrient deficiency using a scalable, high-throughput method. Offers accurate detection with results in under 6 hours and costs less than $1 per sample.
Overview
The Amplification-refractory mutation system (ARMS)-based SNP detection technology offers a rapid, cost-effective, and scalable solution for identifying single nucleotide polymorphisms (SNPs) associated with micronutrient deficiency risks. Utilizing a PCR-based method, it allows for direct detection and differentiation of homozygous wildtype, homozygous mutated, and heterozygous genotypes in a single reaction, with results available in less than 6 hours. This technology is ideal for high-throughput screening applications, particularly in resource-limited settings.
Technical specifications
- Cost-effective: The genomic DNA extraction and SNP detection process costs less than $1 per sample, significantly reducing testing expenses compared to commercial alternatives.
- Rapid results: Capable of processing over 20 samples within a 6-hour timeframe, making it suitable for both commercial and clinical applications.
- High accuracy: Demonstrated 100% accuracy in SNP detection in animal models, validated through sequencing.
- Scalability: Designed to accommodate high-throughput production needs, enhancing its utility in large-scale screening programs.
- Broad application potential: Initially tested in mouse and rat models, with plans to validate using human samples for SNPs linked to conditions such as sickle cell anemia.
Technology readiness level
This technology is currently at TRL 5, having been successfully tested in relevant animal models. Future validation steps include using human samples, particularly those containing SNPs associated with micronutrient deficiencies, to further demonstrate its applicability and reliability.
About University of Tennessee, Knoxville
The University of Tennessee, Knoxville is a comprehensive public land‑grant research university—the flagship of the UT System—classified as R1 and serving more than 40,000 students. Industry engagement is anchored by the UT Research Park at Cherokee Farm, where corporate R&D and joint university–national lab facilities sit just across the river from campus, including assets such as the Volkswagen Innovation Hub and an AT&T 5G testbed. UT’s long‑standing partnership with Oak Ridge National Laboratory—via UT‑Battelle and the UT–Oak Ridge Innovation Institute—gives companies streamlined access to national lab capabilities, talent, and joint programs. A statewide Extension network and established co‑op programs connect companies to faculty expertise and student talent across Tennessee and into federal labs. Research is supported by competitive federal sponsors such as the National Science Foundation and the U.S. Department of Energy. Commercialization is managed by the University of Tennessee Research Foundation, which handles IP, licensing, and startup formation.
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