Aptamer-based biosensors for home detection of vitamin B1 and B2 in urine
Innovative aptamer-based biosensors for home use, designed to detect vitamin B1 and B2 in urine using DNA aptamers and a cell phone-powered detection system.
Overview
This cutting-edge biosensor technology utilizes DNA aptamers to detect vitamin B1 and B2 in urine samples. Aptamers are oligonucleotides that selectively bind to target molecules, making them ideal for sensitive detection. This solution leverages the stability of DNA over RNA to provide a reliable, home-use biosensor platform. The technology is designed for simplicity and accuracy, empowering users to monitor their vitamin levels conveniently at home.
Technical specifications
- Aptamer Selection: DNA aptamers are selected for high affinity to vitamin B1 and B2, building on the lab's extensive experience with over 50 successful target molecule aptamer selections.
- Biosensor Design: A fluorescence-based detection system similar to existing lactate sensors, utilizing a cell phone camera, a 3D-printed adaptor, and a blue LED light source for excitation.
- Dual Detection Capability: The system can simultaneously detect both vitamins using distinct fluorophores, enhancing its utility for comprehensive nutrient monitoring.
Technology readiness level
Currently, this biosensor technology is at Technology Readiness Level 4. The aptamer selection process is underway, with subsequent development and validation of the biosensor's sensitivity and specificity planned. Initial tests will focus on performance in urine samples and the integration of the cell phone-powered detection system.
About University of Waterloo
University of Waterloo is a public research university in Ontario, Canada, known for an entrepreneurial, STEM‑driven culture. A globally recognized co‑op program places students with employers year‑round, creating direct talent pipelines and de‑risked pathways into sponsored research and contract development. An adjacent research and technology park hosts corporate R&D alongside faculty labs, and the campus sits within the Toronto–Waterloo innovation corridor for ready access to partners, investors, and scale‑up resources. Research is supported by competitive federal funding from Canada’s Tri‑Council agencies (NSERC, CIHR, SSHRC) and international programs. A creator‑owned IP policy and a dedicated tech transfer office enable flexible agreements, licensing, and spinouts.
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