Antibiotic-free E. coli expression system for large-scale protein production
An innovative antibiotic-free E. coli expression system engineered for large-scale, cost-effective protein production, offering high yield and genetic stability without antimicrobial resistance (AMR) genes.
Overview
The antibiotic-free E. coli expression system is a breakthrough in biotechnology, enabling large-scale protein production without antimicrobial resistance (AMR) genes. This innovation promises high protein yields exceeding 1 g/L and cost-effective scalability, making it ideal for industrial applications. By eliminating the use of antibiotics, the system mitigates environmental and health concerns associated with AMR gene transfer, ensuring safer and more sustainable protein production processes.
Technical specifications
- Engineered E. coli strain: Free of AMR genes, ensuring no antibiotic resistance is introduced in the production process.
- High yield capacity: Achieves protein production rates of over 1 g/L, supporting large-scale manufacturing needs.
- Scalability: Designed to support industrial-scale bioreactors up to 1000L, maintaining genetic stability and contamination control.
- Purification process: Includes endotoxin removal steps to meet therapeutic standards.
Technology readiness level
This technology is currently at TRL 6, having been validated at a 20L scale with plans for further scaling in collaboration with industry partners. Additional funding is sought for 100L fermentation scale-up and purification processes.
About Intact Genomics
Intact Genomics is an ISO 13485:2016 certified biotechnology company that specializes in the development and manufacture of high-performance molecular biology reagents and genomic services. Founded in 2013 and based in St. Louis, Missouri, the company leverages proprietary technologies, including Fungal Artificial Chromosomes (FAC) and advanced large DNA fragment cloning, to provide solutions for isothermal DNA/RNA amplification, gene editing, and molecular cloning. Their product portfolio, which includes over 100 items such as high-efficiency competent cells, enzymes, and RPA optimization tools, is designed to support high-quality, reproducible research across diverse fields including human health, agriculture, and environmental science.
The company serves a global customer base across academia and industry, enabling researchers to address complex challenges in genomic structure, function, and natural product discovery. By offering both off-the-shelf molecular biology products and custom services—such as library construction, protein expression, and OEM manufacturing—Intact Genomics provides scalable support for diagnostic development and drug discovery efforts. Their commitment to reliability and innovation has established them as a key resource for laboratories seeking specialized tools for streamlined cloning and rapid isothermal testing methods.
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